This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and...
Anecdotal clinical reports of tumor remissions after viral infections laid the foundation for
the field of oncolytic virotherapy. Advances in molecular virology, tumor biology, and
immunology have enabled more refined studies of tumor-selective viruses. Concomitant
with the resurgence of cancer immunotherapy and after...
This volume explores detailed methods and experimental protocols evaluating the effect of a compound or a mixture of compounds on the action of enzymes that are significant targets in pharmaceuticals. Consisting of three sections, the book delves into recent biocomputing and bioinformatics protocols, state-of-the art modern biophysical,...
This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions...
This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors,...
The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL)...
This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion...
This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions....
This detailed book provides state-of-art computational approaches to further explore the exciting opportunities presented by single-cell technologies. Chapters each detail a computational toolbox aimed to overcome a specific challenge in single-cell analysis, such as data normalization, rare cell-type identification, and spatial...
This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and...
This book connects experts in the field of child assessment to provide child psychiatrists with knowledge in evaluation and educational programming. The book provides a review of the latest science behind: common learning disabilities, including etiology and guidelines for assessment/diagnosis; neurodevelopmental disorders, like learning...
This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a...
